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GrainGenes Probe Report: BCD1871

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Probe
BCD1871  [ Marker Report ]
Locus
Xbcd1871-5D
[ Show all 11 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Polymorphism
BCD1871 DraI
BCD1871 EcoRV
BCD1871 EcoRI
BCD1871 HindIII
BCD1871 XbaI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1871 KO autoradiogram
BCD1871 SO autoradiogram
Data Source
Sorrells, Mark E.96.07