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GrainGenes Probe Report: BCD22

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Probe
BCD22  [ Marker Report ]
Locus
BCD22
[ Show all 14 ]
Synonym
SynonymHAR35_15254
HAR35_15255
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438918
BCD22_WHE04E04.s1
Polymorphism
BCD22 HindIII
Linkage Group
1L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
2.1
PCR size
2.1
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD22 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09