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GrainGenes Probe Report: BCD266

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Probe
BCD266  [ Marker Report ]
Locus
[ Hide all but 1 of 9 ]
Xbcd266-2
BCD266
Xcnl.BCD266
BCD266a
BCD266b
XBCD266
Xbcd266-2R
BCD266D
Xbcd266
Synonym
SynonymHAR35_15015
HAR35_15016
HAR35_33212
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438697
BE438789
BCD266_WHE1B0101
BCD266_WHE1B0102
Polymorphism
BCD266 EcoRI
Linkage Group
[b 2L]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.5
PCR size
1.5
Clone Vector
pBluSK
pBluSK-
BS-SK-
lambda_Zap
pBS
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD266 BROWS autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Gill, Bikram S.92.09