Query (optional)   in Class  

GrainGenes Probe Report: BCD351

[ Printable Version ]  [ Submit comment/correction ]

Probe
BCD351  [ Marker Report ]
Locus
BCD351C
[ Show all 31 ]
Synonym
SynonymHAR35_1862
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438985
BE439015
BCD351_WHE05B06.s1
Linkage Group
[b 1H, 2H]
Barley 5 (1H)
4
Barley 7 (5H)
2
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
pBluSK-
pBS
lambda_Zap
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
Graner, Andreas94.03.08
ATMI92.09
Kleinhofs, Andris96.05.08
Gill, Bikram S.92.09