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GrainGenes Probe Report: BCD372

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Probe
BCD372  [ Marker Report ]
Locus
Xbcd372-3A
[ Show all 15 ]
Synonym
SynonymHAR35_15254
HAR35_15255
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438622
BE438665
BE438754
BCD372_WHE1A0071
BCD372_WHE1A0072
Polymorphism
BCD372 EcoRI
Linkage Group
3L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.5
PCR size
1.5
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD372 KO autoradiogram
BCD372 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09