GrainGenes

GrainGenes Probe Report: BCD402

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	BCD402  [ Marker Report ]
Locus	BCD402 [ Show all 17 ]
Synonym	Synonym HAR35_47809 HAR35_7272 HAR35_7906
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Additional faint insert band of PCR_size 1.6. Bar.LambZAPII [c] Bar.LambZAPII
Type	cDNA
Sequence	L44099 BCD402
Polymorphism	BCD402 EcoRI
Linkage Group	[b 4L]
Insert Enzyme	EcoRI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
Insert Size	3.1
PCR size	3.1
Clone Vector	pBluSK pBluSK- BS-SK-
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E.
Clone Authority	Sorrells, Mark E.
Image	BCD402 BROWS autoradiogram BCD402 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09

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