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GrainGenes Probe Report: BCD453

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Probe
BCD453  [ Marker Report ]
Locus
[ Hide all but 1 of 14 ]
Xbcd453-2B
BCD453
Xcnl.BCD453
BCD453A
BCD453B
BCD453B(69.8)
Xbcd453
BCD453c
BCD453d
BCD453b-4H
BCD453(A)
BCD453(B)
BCD453(C)
BCD453(D)
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438630
BE438783
BCD453_WHE1E0081
BCD453_WHE1E0082
Linkage Group
[b 2L]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.7
PCR size
0.7
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09