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GrainGenes Probe Report: BCD808

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Probe
BCD808  [ Marker Report ]
Locus
Xbcd808-1
[ Show all 46 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438617
BE438742
BCD808_WHE1G0031
BCD808_WHE1G0032
Polymorphism
BCD808 EcoRV
BCD808 EcoRI
Linkage Group
1L, 4L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.8
PCR size
0.8
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD808 KO autoradiogram
BCD808 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09