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GrainGenes Probe Report: BCD828

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Probe
BCD828  [ Marker Report ]
Locus
Xbcd828-3
[ Show all 11 ]
Synonym
Other nameBCD1127
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
STS
PCR primers
AACAGCTATGACCATG
GTAAAACGACGGCCACT
Sequence
BE438720
BE438812
BCD828_WHE1D0101
BCD828_WHE1D0102
BCD828-5
Polymorphism
BCD828 EcoRI
BCD828 XbaI
Linkage Group
3L
3
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.7
PCR size
0.7
Clone Vector
pBluSK
pBluSK-
BS-SK-
pBS
lambda_Zap
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Blake, Tom
Image
BCD828 BROWS autoradiogram
BCD828 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
[ Show all 6 ]

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