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GrainGenes Probe Report: BCD880

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Probe
BCD880  [ Marker Report ]
Locus
Xbcd880-6A
[ Show all 10 ]
Synonym
Please seeBCD1510
SynonymHAR35_15570
Related Probe
BCD1510
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Based on surveys and mapping in Chinese Spring aneuploids and the wheat Synthetic x Opata population, this probe appears to be identical to BCD1510, and should be referred to by that name. (Clare Nelson, August 1995)
Type
cDNA
Sequence
BE438921
BCD880_WHE04A06.s1
Polymorphism
BCD880 EcoRV
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD880 KO autoradiogram
BCD880 SO autoradiogram
Data Source
Sorrells, Mark E.96.07