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GrainGenes Probe Report: BCD9

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Probe
BCD9  [ Marker Report ]
Locus
Xbcd9-5B
[ Show all 8 ]
Synonym
SynonymHAR35_751
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438616
BE438740
BCD9_WHE1F0041
BCD9_WHE1F0042
Polymorphism
BCD9 EcoRI
BCD9 EcoRV
BCD9 HindIII
BCD9 DraI
BCD9 XbaI
Linkage Group
5L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.1
PCR size
1.1
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD9 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09