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GrainGenes Probe Report: BCD926

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Probe
BCD926  [ Marker Report ]
Locus
Xbcd926-5A
[ Show all 7 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Polymorphism
BCD926 EcoRI
Durums BCD926 EcoRI
BCD926 HindIII
Linkage Group
5L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
BCD926 BROWS autoradiogram
BCD926 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

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