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GrainGenes Probe Report: BCD961

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Probe
BCD961  [ Marker Report ]
Locus
Xbcd961
bcd961
BCD961
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438631
BE438681
BE438772
BCD961_WHE1F0081
BCD961_WHE1F0082
Polymorphism
BCD961 EcoRI
BCD961 EcoRV
BCD961 DraI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD961 KO autoradiogram
Data Source
Sorrells, Mark E.96.07