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GrainGenes Probe Report: BE495011

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Probe
BE495011  [ Marker Report ]
Locus
HI058D8
HI056P24
D1.1_ctg5733
Yr48
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
ReferenceHegarty J et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr48 MAS Wheat. Marker Assisted Selection in Wheat.
PCR primers
BE495011-F 5'- TGA TTA CTG TAG CTA CCT CCT CCT
BE495011-R 5'- GGT GCA AGA TGT GCC TGT AA
Amplification Conditions
Denaturing step: 94C, 3 min. Amplification step (40 cycles): 95C, 30 sec; 60C, 30 sec; 72C, 30 sec. Extensions step: 72C, 5 min
Source Species
Triticum aestivum