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GrainGenes Probe Report: Bmag121

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
Bmag121  [ Marker Report ]
Locus
Bmag0121b
Bmag0121c
Bmag0121a
Bmag121a
Synonym
Other nameBmag0121
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)21/(AC)12
Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type
PCR
SSR
PCR primers
5' ATAAGATAGGTCACCGCAATA 3'
5' AGTAGTTCAATACAGACCTACAGG 3'
SSR size
171
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare

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