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GrainGenes Probe Report: Bmag174

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Probe
Bmag174  [ Marker Report ]
Locus
Bmag0174a
Bmag0174b
Bmag0174
Synonym
Other nameBmag0174
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)9
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR
SSR
PCR primers
5' AATTTGCTGAAGCTAGCAG 3'
5' GCGATCTCTTTCCAACTG 3'
SSR size
143
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare