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GrainGenes Probe Report: Bmag345

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
Bmag345  [ Marker Report ]
Locus
Bmag0345
Bmag345
Bmag345a
Bmag345b
Synonym
Other nameBmag0345
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (CT)6AA(CT)9AA(CT)6AA(CT)7AA(CT)7
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.29
Type
PCR
SSR
PCR primers
5' ATGAGGAATAACTCAACCAAA 3'
5' AATATATTTTCGATGTCGAGC 3'
SSR size
140
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare

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