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GrainGenes Probe Report: Bmag369

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Probe
Bmag369  [ Marker Report ]
Locus
Bmag0369
Bmag369
Synonym
Other nameBmag0369
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (CT)16
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.81
Type
PCR
SSR
PCR primers
5' CACTAGGCACCAATGACTG 3'
5' ATCGAAAATCTTAGCTTTGG 3'
SSR size
191
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare