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GrainGenes Probe Report: Bmag603

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Probe
Bmag603  [ Marker Report ]
Locus
Bmag0603
Bmag603
Synonym
Other nameBmag0603
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)24
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.78
Type
PCR
SSR
PCR primers
5' ATACCATGATACATCACATCG 3'
5' GGGGGTATGTACGACTAACTA 3'
ATACCATGATACATCACATCG
GGGGGTATGTACGACTAACTA
SSR size
120
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare