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GrainGenes Probe Report: Bmgtttttt1

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Probe
Bmgtttttt1  [ Marker Report ]
Locus
Bmgtttttt0001
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (GTTTTT)5
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.5
Type
PCR
SSR
PCR primers
5' ACACCAGAGCCTTGACTCGT 3'
5' AGCAGCAACAACGACAACAC 3'
SSR size
215
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 69C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 68C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 63C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare