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GrainGenes Probe Report: CDO1160

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Probe
CDO1160  [ Marker Report ]
Locus
cdo1160
[ Show all 7 ]
Synonym
SynonymHAR35_48153
HAR35_49183
HAR35_730
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
AA231697
AA231698
CDO1160-5
CDO1160-3
Polymorphism
CDO1160 EcoRI
CDO1160 EcoRV
CDO1160 DraI
CDO1160 XbaI
Linkage Group
1L, 3L
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.3
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO1160 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09