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GrainGenes Probe Report: CDO1196

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Probe
CDO1196  [ Marker Report ]
Locus
Xcdo1196
[ Show all 10 ]
Synonym
SynonymHAR35_14205
HAR35_14207
HAR35_42740
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439362
BE439331
CDO1196_WHE05E10.s1
Polymorphism
CDO1196 DraI
CDO1196 EcoRI
CDO1196 EcoRV
CDO1196 HindIII
CDO1196 XbaI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO1196 KO autoradiogram
CDO1196 SO autoradiogram
Data Source
Sorrells, Mark E.96.07