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GrainGenes Probe Report: CDO1379

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Probe
CDO1379  [ Marker Report ]
Locus
Xcdo1379-2D
Xcdo1379
cdo1379
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Polymorphism
CDO1379 XbaI
CDO1379 EcoRI
CDO1379 EcoRV
CDO1379 HindIII
CDO1379 DraI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
0.9
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO1379 SO autoradiogram
CDO1379 SO autoradiogram #2
Data Source
Sorrells, Mark E.96.07