Query (optional)   in Class  

GrainGenes Probe Report: CDO1421

[ Printable Version ]  [ Submit comment/correction ]

Probe
CDO1421  [ Marker Report ]
Locus
Xcdo1421-6B
Xcdo1421
Related Probe
BCD1495
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439203
CDO1421_WHE03E02.r1
Polymorphism
CDO1421 EcoRI
CDO1421 EcoRV
CDO1421 DraI
CDO1421 XbaI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
0.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO1421 SO autoradiogram
Data Source
Sorrells, Mark E.96.07