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GrainGenes Probe Report: CDO1502

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Probe
CDO1502  [ Marker Report ]
Locus
Xcdo1502
[ Show all 10 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Oat Uni-ZapXR
Type
cDNA
Amplification Conditions
94 C/1 min, 50 C/40 sec, 72 C/1 min, 34 cycles, 72 C/10 min
Polymorphism
Durums CDO1502 EcoRI
CDO1502 EcoRI
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
etiolated leaf tissue
Insert Size
1.4
PCR size
1.3
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
M13 forward and reverse
Sk and T7
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
Crown rust resistance markers
CDO1502 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
Bush, Arla L.95.05