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GrainGenes Probe Report: CDO281

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
CDO281  [ Marker Report ]
Locus
Xcdo281-3A
[ Show all 6 ]
Synonym
SynonymHAR35_20302
HAR35_3906
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439284
BE439293
CDO281_WHE04G10.s1
Polymorphism
CDO281 DraI
CDO281 XbaI
CDO281 EcoRI
CDO281 EcoRV
CDO281 HindIII
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
PCR size
2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO281 SO autoradiogram
Data Source
Sorrells, Mark E.96.07

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