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GrainGenes Probe Report: CDO370

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Probe
CDO370  [ Marker Report ]
Locus
Xcdo370-2
[ Show all 6 ]
Synonym
SynonymHAR35_14503
HAR35_14504
HAR35_29816
External Databases
DDBJ
EMBL
GenBank
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
STS
PCR primers
GCT CTC CGC CTG GAT ACA GC
CCC TTG CCC TTC TTC TTC TG
Sequence
BE439107
CDO370_WHE2E0051
CDO370_WHE2E0052
CDO370
Polymorphism
CDO370 EcoRI
CDO370 EcoRV
CDO370 DraI
Linkage Group
[b 2LS]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1
PCR size
1
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Blake, Tom
Image
CDO370 BROWS autoradiogram
CDO370 KO autoradiogram
CDO370 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
Blake, Tom95.08.24
ATMI92.09