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GrainGenes Probe Report: CDO395

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Probe
CDO395  [ Marker Report ]
Locus
cdo395
[ Show all 16 ]
Synonym
SynonymHAR35_16251
HAR35_16252
HAR35_29914
External Databases
DDBJ
EMBL
GenBank
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
STS
PCR primers
ACC CCA TCG GTC TGT CTA TG
AAC TCG GTC GTT ACA AGA GC
Sequence
BE439071
[ Show all 7 ]
Polymorphism
CDO395 EcoRI
Durums CDO395 EcoRI
CDO395 EcoRV
Linkage Group
3S [b 3S]
3S [b 3S]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1
PCR size
1
Clone Vector
pBluSK
BS-SK-
lambda-ZapXR
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Blake, Tom
Image
CDO395 BROWS autoradiogram
CDO395 KO autoradiogram
CDO395 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
Blake, Tom95.08.24
ATMI92.09
Gill, Bikram S.92.09