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GrainGenes Probe Report: CDO400

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Probe
CDO400  [ Marker Report ]
Locus
CDO400b
[ Show all 12 ]
Synonym
SynonymHAR35_19904
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439060
[ Show all 6 ]
Polymorphism
CDO400 DraI
Linkage Group
1L, 5L
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
0.8
PCR size
0.8
Clone Vector
pBluSK
lambda-ZapXR
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO400 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Gill, Bikram S.92.09