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GrainGenes Probe Report: CDO405

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Probe
CDO405  [ Marker Report ]
Locus
CDO405
[ Show all 18 ]
Synonym
SynonymHAR35_3670
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
BE439061
[ Show all 6 ]
Polymorphism
CDO405 EcoRI
Durums CDO405 EcoRI
CDO405 EcoRV
Linkage Group
2S
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.8
PCR size
1.8
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO405 BROWS autoradiogram
CDO405 KO autoradiogram
CDO405 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09