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GrainGenes Probe Report: CDO455

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Probe
CDO455  [ Marker Report ]
Locus
CDO455b
[ Show all 6 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
AA231702
AA231871
Polymorphism
CDO455 EcoRI
Linkage Group
3L
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
Insert Size
1.2
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO455 BROWS autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

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