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GrainGenes Probe Report: CDO456

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Probe
CDO456  [ Marker Report ]
Locus
Xcdo456-2
[ Show all 15 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439270
CDO456_WHE04F07.s1
AA231913
AA231914
Polymorphism
CDO456 EcoRI
CDO456 DraI
CDO456 HindIII
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO456 BROWS autoradiogram
CDO456 KO autoradiogram
CDO456 SO autoradiogram
Data Source
Sorrells, Mark E.96.07