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GrainGenes Probe Report: CDO457

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Probe
CDO457  [ Marker Report ]
Locus
[ Hide all but 1 of 14 ]
CDO457a
CDO457
Xcdo457A
Xcdo457-5A
Xcnl.CDO457
Xcdo457
Xcdo457B
Xcdo457-5B
cdo457b
Xcdo457-4A
cdo457
CDO457A
CDO457B
cdo457-5A
Synonym
SynonymHAR35_20530
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439076
[ Show all 6 ]
Polymorphism
CDO457 EcoRI
CDO457 EcoRV
CDO457 HindIII
CDO457 XbaI
Linkage Group
[b 7L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO457 BROWS autoradiogram
CDO457 KO autoradiogram
CDO457 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09