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GrainGenes Probe Report: CDO460

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Probe
CDO460  [ Marker Report ]
Locus
cdo460
[ Show all 21 ]
Synonym
SynonymHAR35_10115
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439048
[ Show all 6 ]
Polymorphism
CDO460 DraI
CDO460 EcoRI
CDO460 XbaI
Linkage Group
[b 7LS]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.2
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO460 KO autoradiogram
CDO460 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09