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GrainGenes Probe Report: CDO465

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Probe
CDO465  [ Marker Report ]
Locus
[ Hide all but 1 of 12 ]
CDO465
Xcnl.CDO465
HD004N5
HD027P3
HI003L4
HI013P14
Xcdo465
Xcdo465-5A
Xcdo465d
Xcdo465b
Xcdo465-5B
cdo465xp
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439135
CDO465_WHE2G0092
CDO465_WHE2G0091
Linkage Group
[b 4L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.8
PCR size
1.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09