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GrainGenes Probe Report: CDO484

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Probe
CDO484  [ Marker Report ]
Locus
CDO484
[ Show all 18 ]
Synonym
SynonymHAR35_16073
HAR35_16075
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
BE439064
[ Show all 6 ]
Polymorphism
CDO484 EcoRI
CDO484 EcoRV
Linkage Group
2DL, 4AL, 5BL,
2L4L5L[b 7L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.6
PCR size
1.6
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO484 BROWS autoradiogram
CDO484 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09