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GrainGenes Probe Report: CDO504

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Probe
CDO504  [ Marker Report ]
Locus
CDO504
[ Show all 8 ]
Synonym
SynonymHAR35_17977
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
BE439141
CDO504_WHE2E0121
CDO504_WHE2E0122
CDO504-5
Polymorphism
CDO504 EcoRI
CDO504 EcoRV
CDO504 HindIII
CDO504 DraI
CDO504 XbaI
Linkage Group
[b 7L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
0.8
PCR size
0.8
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO504 BROWSSM autoradiogram
CDO504 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09