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GrainGenes Probe Report: CDO507

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
CDO507  [ Marker Report ]
Locus
CDO507a
[ Show all 11 ]
Synonym
SynonymHAR35_14437
HAR35_38536
HAR35_47154
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439279
[ Show all 11 ]
Polymorphism
CDO507 EcoRI
CDO507 EcoRV
CDO507 HindIII
CDO507 DraI
CDO507 XbaI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
PCR size
2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO507 BROWS autoradiogram
CDO507 SO autoradiogram
Data Source
Sorrells, Mark E.96.07

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