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GrainGenes Probe Report: CDO520

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Probe
CDO520  [ Marker Report ]
Locus
Xcdo520b
Xcdo520a
CDO520
Xcdo520-5D
Xcdo520-5A
Synonym
SynonymHAR35_19896
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439045
[ Show all 6 ]
Polymorphism
CDO520 EcoRI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.6
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO520 BROWS autoradiogram
Data Source
Sorrells, Mark E.96.07