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GrainGenes Probe Report: CDO537

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Probe
CDO537  [ Marker Report ]
Locus
CDO537
Xcdo537-2
Xcnl.CDO537
Synonym
SynonymHAR35_18816
HAR35_21741
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439137
CDO537_WHE2H0091
CDO537_WHE2H0092
CDO537-5
CDO537-3
Linkage Group
[b 2LS]
2
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
2.1
PCR size
2.1
Clone Vector
pBluSK
pBS
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Kleinhofs, Andris96.05.08