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GrainGenes Probe Report: CDO54

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Probe
CDO54  [ Marker Report ]
Locus
Xcdo54-3
[ Show all 7 ]
Synonym
SynonymHAR35_3418
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439052
[ Show all 6 ]
Polymorphism
CDO54 EcoRI
CDO54 EcoRV
CDO54 DraI
CDO54 HindIII
CDO54 XbaI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
PCR size
1.9
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO54 KO autoradiogram
CDO54 SO autoradiogram
Data Source
Sorrells, Mark E.96.07

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