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GrainGenes Probe Report: CDO551

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Probe
CDO551  [ Marker Report ]
Locus
Xcdo551-7B
[ Show all 6 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Polymorphism
CDO551 EcoRV
Linkage Group
7L
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
2.5
PCR size
2.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO551 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09