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GrainGenes Probe Report: CDO608

[Submit comment/correction] [What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
CDO608  [ Marker Report ]
Locus
Xcdo608A
[ Show all 15 ]
Reference
ReferenceWight CP et al. (2004) Discovery, localization, and sequence characterization of molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) Molecular Breeding 14:349-361.
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
CN180778
AI978378
AI978377
Polymorphism
CDO608 DraI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO608 KO autoradiogram
Data Source
Sorrells, Mark E.96.07

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