Query (optional)   in Class  

GrainGenes Probe Report: CDO638

[ Printable Version ]  [ Submit comment/correction ]

Probe
CDO638  [ Marker Report ]
Locus
Xcdo638-3A
[ Show all 6 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439278
CDO638_WHE04H08.s1
Polymorphism
CDO638 EcoRV
CDO638 XbaI
CDO638 EcoRI
CDO638 HindIII
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.4
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO638 KO autoradiogram
CDO638 SO autoradiogram
Data Source
Sorrells, Mark E.96.07