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GrainGenes Probe Report: CDO64

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Probe
CDO64  [ Marker Report ]
Locus
Xcdo64-2
[ Show all 11 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
BE439283
CDO64_WHE04F12.s1
CDO64-5
Polymorphism
CDO64 EcoRI
Linkage Group
2S [b 2S]
2S [b 2S]
2
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
BS-SK-
pBS
lambda-ZapXR
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO64 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Kleinhofs, Andris96.05.08
Gill, Bikram S.92.09