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GrainGenes Probe Report: CDO673

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Probe
CDO673  [ Marker Report ]
Locus
CDO673
[ Show all 9 ]
Synonym
SynonymHAR35_5301
External Databases
DDBJ
EMBL
GenBank
Reference
ReferenceWight CP et al. (2004) Discovery, localization, and sequence characterization of molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) Molecular Breeding 14:349-361.
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
STS
PCR primers
GCC ATT GGT ATT GCG AGA GG
ACG AGC GTC AGT CGG TCA CT
Sequence
BE439193
[ Show all 8 ]
Linkage Group
1LS
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.1
PCR size
1.1
Clone Vector
pBluSK
lambda-ZapXR
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Blake, Tom
Image
CDO673 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
Blake, Tom95.08.24
ATMI92.09
Gill, Bikram S.92.09