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GrainGenes Probe Report: CDO677

Probe
CDO677  [ Marker Report ]
Locus
Xcdo677
[ Show all 10 ]
Synonym
SynonymHAR35_11857
HAR35_28766
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439194
CDO677_WHE03B02.r1
Polymorphism
CDO677 EcoRI
CDO677 EcoRV
CDO677 DraI
Linkage Group
5S
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Triticum aestivum
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.5
PCR size
1.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO677 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Smith-White, Brian