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GrainGenes Probe Report: CDO770

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Probe
CDO770  [ Marker Report ]
Locus
CDO770
[ Show all 9 ]
Synonym
SynonymHAR35_16656
HAR35_16657
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
STS
PCR primers
AAT TTA CAG CAA TCA TTT CA
AAG TAG TTG GAA GAA AGT TA
Sequence
BE439157
BE439226
CDO770_WHE03D07.r1
CDO770_WHE03D07.s1
CDO770
Polymorphism
CDO770 EcoRI
CDO770 DraI
Linkage Group
[b 2LS]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Blake, Tom
Image
CDO770 BROWS autoradiogram
CDO770 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
Blake, Tom95.08.24
ATMI92.09