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GrainGenes Probe Report: CDO795

Probe
CDO795  [ Marker Report ]
Locus
CDO795
[ Show all 13 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
AA231718
AA231719
Polymorphism
CDO795 EcoRI
CDO795 EcoRV
Linkage Group
[b 4L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.5
PCR size
1.5
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO795 BROWS autoradiogram
CDO795 KO autoradiogram
CDO795 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09