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GrainGenes Probe Report: CDO92

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
CDO92  [ Marker Report ]
Locus
Xcdo92-1A
[ Show all 8 ]
Synonym
SynonymHAR35_17303
HAR35_17304
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439262
CDO92_WHE04G02.s1
Polymorphism
CDO92 EcoRI
Linkage Group
2S
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO92 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

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